ACUTE TOXICITY STUDIES AND EVALUATION OF ANALGESIC PROPERTY OF TECTONA GRANDIS METHANOLIC SEED EXTRACT IN SWISS ALBINO MICE

Objective: Tectona grandis which is well known as teak possesses a wide range of applications in Indian and African folklore medicine. All the plant parts are having diverse bioactivities, especially the seeds, having hair growth-promoting activity, anti-inflammatory, and antimicrobial activity. Xanthones, proteins, amino acids, and fatty acids have been isolated from the oil and seeds of T. grandis. Methods: The present study was to explore the antinociceptive properties of methanolic seed extract of T. grandis. Dried seeds of the plant were defatted with non-polar solvents such as petroleum ether and extracted with methanol. Acetic acid-induced writhing test, tail flick test, and tail immersion test were employed for the extract using Swiss albino mice. Results: From the results, it is clear that the methanolic teak seed extract is safe at 1000 mg/kg and having a potent analgesic property (at a dose of 200–250 mg/kg body weight) by inhibiting pain response time. Conclusion: It can be concluded that the methanolic seed extract of T. grandis is analgesic in nature. It is considered as safe and the activity may be due to the presence of various bioactive chemicals such as flavonoids, xanthones, and glycosides

Comparison on electrical properties of pure Y2O3, bismuth doped Y2O3, zinc doped Y2O3, bismuth and zinc codoped Y2O3 prepared by sol-gel method

Yttrium oxide is widely studied nowadays as a substitute for SiO2 which is a well-known dielectric material. The uniqueness of yttrium oxide is that it has a high melting point (2430 °C) which is higher than other host materials like alumina, zirconia and yttrium aluminium garnet. Hence an attempt was made to add the dopants, bismuth and zinc simultaneously to the yttrium oxide lattice and to study their properties as a dielectric material. Also the dependence of their electrical properties on structure, particle size and morphology is analysed. Pure Yttrium oxide, bismuth doped yttrium oxide, zinc doped yttrium oxide and bismuth and zinc codoped yttrium oxide samples of 0.5 weight percentage of yttrium precursor as dopant concentration are synthesized by simple precipitation technique, sol-gel method. Electrical properties of the samples are studied and a comparative study is done on the doped and codoped samples and it was found that codoped samples show better electrical properties compared with single dopant samples.

Case report on perioperative management of combined factor V and VIII deficiency in a patient with prolapse uterus

Combined factor V and factor VIII deficiency is a rare autosomal recessive disorder with incidence of one in 10,00,000. We reported a case of seventy-one-year-old patient with third degree uterovaginal prolapse, diagnosed as combined factor V and VIII deficiency and the successful perioperative management during vaginal hysterectomy. With our experience in this, we conclude that for the patient's with factor V and VIII deficiency that the correction should be given for at least 2 weeks post operatively

UV SPECTROPHOTOMETRIC ASSAY METHOD DEVELOPMENT AND VALIDATION OF DABIGATRAN ETEXILATE IN CAPSULES

Objective: To develop a simple and a cheap UV spectrophotometric method for the quantitative estimation of Dabigatran etexilate in capsules and validate as per ICH guidelines.Methods: The optimized method uses triethyl ammonium phosphate aqueous solution (pH 2.5) as a solvent for the estimation of assay of Dabigatran etexilate in capsules at a wavelength of 325 nm.Results: The developed method resulted in Dabigatran etexilate exhibiting linearity in the range 5-15μg/ml. The assay precision is exemplified by relative standard deviation of 1.07%. Percentage Mean recovery was found to be in the range of 98â€102, during accuracy studies. Method was found to be robust with respect to wavelength and pH of the solvent. Conclusion: A simple and a cheap UV spectrophotometric method was developed and validated for the quantitative estimation of Dabigatran etexilate in capsules as per ICH guidelines and hence it can be used for routine analysis in various pharmaceutical industries.Â

LEAKAGE POWER REDUCTION IN CMOS CIRCUITS USING LEAKAGE CONTROL TRANSISTOR TECHNIQUE IN NANOSCALE TECHNOLOGY

In CMOS circuits, as the technology scales down to nanoscale, the sub-threshold leakage current increases with the decrease in the threshold voltage. LECTOR, a technique to tackle the leakage problem in CMOS circuits, uses two additional leakage control transistors, which are self-controlled, in a path from supply to ground which provides the additional resistance thereby reducing the leakage current in the path. The main advantage as compared to other techniques which involves the sleep transistor is that LECTOR technique does not require any additional control and monitoring circuitry, thereby limits the area increase and also the power dissipation in active state. Along with this, the other advantage with LECTOR technique is that it does not affect the dynamic power which is the major limitation with the other leakage reduction techniques

Paper Session II-A - Mixed-Mode Interfacial Fracture Toughness of Sandwich Composites at Cryogenic Temperatures

Honeycomb sandwich composites are found in a wide range of structural applications due to their high strength and stiffness-to-weight ratio compared to other systems. Current use of sandwich composites ranges from secondary structures in commercial aircrafts to primary structures in military aircraft, helicopters, and reusable launch vehicles, e.g. Space Shuttle. One of the applications of sandwich construction is in the liquid hydrogen tank of future RL V\u27s. Because of their low density and high stiffness sandwich construction is attractive for LH2 tank. However, past tests shave shown that leakage of hydrogen through the composite face sheet and subsequent de bonding of the face-sheet is a major concern in using sandwich construction. This problem can be eliminated by thorough understanding of the fracture mechanics of face sheets under cryogenic conditions. This study aimed to understand the failure phenomena of sandwich composites constructed from carbon fiber/epoxy composite face sheets and Nomex honeycomb cores. Both experiments including testing ·under cryogenic conditions and finite element analyses are performed to understand the conditions under which debonding occurs and propagates. One of the major objectives of the study is to measure the critical energy release rate or fracture toughness of the face-sheet/core interface, which will be a strong function of mode-mixity and temperature. Furthermore, mode-mixity itself will depend up on the geometric factors such as crack length, face sheet and core thickness, and material stiffness parameters. Fracture tests similar to double cantilever beams will be performed on sandwich panels containing initial delaminations. The fracture toughness will be measured for various crack lengths. The loads at which crack propagation occurs will be applied in the finite element model of the panel to obtain the detailed stress field in the vicinity of the crack tip. From the results of the fracture tests and finite element analysis the interfacial fracture toughness of the sandwich panel under cryogenic conditions can be measured. Application of the results to the design of a LH2 tank will be demonstrated

Studies of the genetics of inheritance of stem rust resistance in bread wheat

Pgt race TTKSK (Ug99) has a wide virulence range with respect to currently grown wheat cultivars worldwide. Aspects of migration, mutation, recombination and selection in the pathogen have led to previously deployed stem rust resistance genes being ineffective. Race TTKSK has further evolved to acquire virulence for resistance genes such as Sr24 (race Ug99 + Sr24) and Sr36 (Ug99 + Sr36). Five resistant wheat lines (KSL-2, KSL-3, KSL-5, KSL-12 and KSL-19) which were resistant in tests during 2008, 2009 and 2010 were used as parents in crosses with stem rust susceptible line CACUKE to develop genetic populations for determining the inheritance of resistance to stem rust. F3 populations were evaluated at KARI Njoro in the 2012 off season and 2012 to 2013 main season. The adult plant stem rust responses were scored using the modified Cobb’s scale. The F2:3 lines of population(s) exhibiting qualitative variation were grouped as homozygous resistant (HR), segregating (Seg) and homozygous susceptible (HS). Heavy disease pressure was present during the cropping seasons with the check CACUKE displaying 90% susceptibility. Chi square analysis revealed that the segregation data in the parent KSL-2 did not deviate significantly from the single gene model (1:2:1) suggesting that the resistance to stem rust is conditioned by a single dominant gene. The Chi square test also revealed that the stem rust resistance in the parents KSL-3, KSL-5, KSL-12 and KSL-19 was conditioned by two genes. The families from the KSL-2 and KSL-3 crosses also segregated for the presence of the pseudo black chaff implying that the Sr2 gene could be present in the background of these wheat parents. The superior transgressive segregants identified in these crosses will be used in breeding.Keywords: Genetic, wheat, gene, resistanceAfrican Journal of Biotechnology Vol. 12(21), pp. 3153-315

DELAYED MITOGENIC STIMULATION DECREASES DNA DAMAGE ASSESSED BY MICRONUCLEUS ASSAY IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES AFTER 60CO IRRADIATION

While contradictory reports are available on the yield of dicentric chromosomes (DC) in blood samples stored at different temperature and stimulated to enter into cell cycle, various times gap followed by exposure, limited information is available on the micronucleus (MN) assay. As scoring the micronuclei frequency from the blood lymphocytes of exposed individuals is an alternative to the gold standard DC assay for triage applications, we examined radiation induced MN yield in delayed mitogenic stimulation after irradiation of in vitro. Peripheral blood lymphocytes (PBL) were exposed to low LET (60Co) radiation dose (0.1 to 5Gy) and incubated at 37°C for 2, 6 and 24 hours. The MN frequency obtained in blood samples stimulated 2 hours post-irradiation showed a dose dependent increase and used to construct the dose-response curve. Further, the results also showed that blood samples stimulated twenty four hours of post-irradiation, a significant reduction (p\u3c0.05) in MN frequencies were obtained when compared to that of blood samples stimulated two hours and six hours after post-irradiation (0.5, 1, 3 and 5Gy). The observed result suggests that the prolonged PBL storage without mitogenic stimulation could lead to interphase cell death and a delayed blood sampling could results in underestimation of dose in biological dosimetry

Development of microsatellite markers for the resin-yielding, non-timber forest product species Boswellia serrata (Burseraceae)

PREMISE OF THE STUDY: Boswellia serrata (Burseraceae) is an economically important aromatic,gum-resin–yielding, non-timber forest tree species. Microsatellite markers were developed for B. serrata for the first time to study genetic diversity and population structure. METHODS AND RESULTS: A magnetic bead enrichment method was used to develop 16 microsatellite markers, of which 11 were polymorphic. The number of alleles per locus in the 60 individuals studied ranged from three to 10, and the levels of observed and expected heterozygosity ranged from 0.50 to 0.90 and 0.666 to 0.861, respectively. The primers successfully amplified in the congeneric species B. ovalifoliolata. CONCLUSIONS: These microsatellite markers can be used to study the genetic variation and population structure of B. serrata and to provide crucial information on population and ecological issues for management and conservation of the species